Importance of pre-analytical factors contributing to measurement uncertainty, when determining sulfadoxine and sulfamethoxazole from capillary blood dried on sampling paper.
Document identifier: oai:dalea.du.se:2668
Keyword: Natural Sciences,
Chemical Sciences,
Naturvetenskap,
KemiPublication year: 2008Abstract: A bioanalytical method is developed and validated for determination of sulfadoxine (SD) and sulfamethoxazole (SM) in 100 µL capillary blood dried on sampling paper (Whatman 31ET Chr). SD and SM are extracted with 2000 µL perchloric acid and the liquid phase is loaded onto ENV+ solid-phase extraction columns. SD, SM, and the internal standard are separated on a Purospher STAR RP-18 liquid chromatography column (150 × 4.6 mm) with a mobile phase consisting of acetonitrile–sodium acetate buffer pH 5.2, I = 0.1 (33:67, v/v). Analytes are detected with UV at 256 nm. Lower limit of quantitation is 5 µmol/L, where precisions are 4.2% and 3.9% for SD and SM, respectively. Three brands of sampling papers have been compared with respect to absorption properties, extraction recoveries, and variations. Punching out dried blood spots (DBS) instead of cutting spots into strips prior to extraction has been evaluated by examining precision and accuracy of SD and SM determinations. Importance of uniformity of types of sampling paper, sampling volume and biological matrix, benefit of punching out discs from DBS, and impact on absorption properties of different brands of sampling papers are discussed. Avoiding pre-analytical errors whenever possible results in concentrations determined being more accurate and precise.
Authors
Mikaela Malm
Högskolan Dalarna; Kemiteknik
Other publications
>>
Jenny Lindkvist
Other publications
>>
Yngve Bergqvist
Högskolan Dalarna; Kemiteknik
Other publications
>>
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header:
identifier: oai:dalea.du.se:2668
datestamp: 2021-04-15T12:52:55Z
setSpec: SwePub-du
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recordCreationDate: 2007-04-04
identifier:
http://urn.kb.se/resolve?urn=urn:nbn:se:du-2668
19007488
titleInfo:
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lang: eng
title: Importance of pre-analytical factors contributing to measurement uncertainty when determining sulfadoxine and sulfamethoxazole from capillary blood dried on sampling paper.
abstract: A bioanalytical method is developed and validated for determination of sulfadoxine (SD) and sulfamethoxazole (SM) in 100 µL capillary blood dried on sampling paper (Whatman 31ET Chr). SD and SM are extracted with 2000 µL perchloric acid and the liquid phase is loaded onto ENV+ solid-phase extraction columns. SD SM and the internal standard are separated on a Purospher STAR RP-18 liquid chromatography column (150 × 4.6 mm) with a mobile phase consisting of acetonitrile–sodium acetate buffer pH 5.2 I = 0.1 (33:67 v/v). Analytes are detected with UV at 256 nm. Lower limit of quantitation is 5 µmol/L where precisions are 4.2% and 3.9% for SD and SM respectively. Three brands of sampling papers have been compared with respect to absorption properties extraction recoveries and variations. Punching out dried blood spots (DBS) instead of cutting spots into strips prior to extraction has been evaluated by examining precision and accuracy of SD and SM determinations. Importance of uniformity of types of sampling paper sampling volume and biological matrix benefit of punching out discs from DBS and impact on absorption properties of different brands of sampling papers are discussed. Avoiding pre-analytical errors whenever possible results in concentrations determined being more accurate and precise.
subject:
@attributes:
lang: eng
authority: uka.se
topic:
Natural Sciences
Chemical Sciences
@attributes:
lang: swe
authority: uka.se
topic:
Naturvetenskap
Kemi
language:
languageTerm: eng
genre:
publication/journal-article
ref
note:
Published
3
name:
@attributes:
type: personal
authority: du
namePart:
Malm
Mikaela
role:
roleTerm: aut
affiliation:
Högskolan Dalarna
Kemiteknik
nameIdentifier: mml
@attributes:
type: personal
namePart:
Lindkvist
Jenny
role:
roleTerm: aut
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type: personal
authority: du
namePart:
Bergqvist
Yngve
role:
roleTerm: aut
affiliation:
Högskolan Dalarna
Kemiteknik
nameIdentifier: ybq
originInfo:
dateIssued: 2008
relatedItem:
@attributes:
type: host
titleInfo:
title: Journal of Chromatographic Science
identifier:
0021-9665
1945-239X
part:
detail:
@attributes:
type: volume
number: 46
@attributes:
type: issue
number: 9
extent:
start: 837
end: 843
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