Straightforward and rapid determination of sulfadoxine and sulfamethoxazole in capillary blood on sampling paper with liquid chromatography and UV detection
Document identifier: oai:dalea.du.se:2659
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10.1016/j.trstmh.2008.11.031Keyword: Engineering and Technology,
Chemical Engineering,
Teknik och teknologier,
Kemiteknik,
Malaria; Pneumonia; Sulfadoxine; Sulfamethoxazole; Chromatography; Dried blood spotsPublication year: 2009Relevant Sustainable Development Goals (SDGs):
The SDG label(s) above have been assigned by OSDG.aiAbstract: A method for the determination of sulfadoxine and sulfamethoxazole in capillary blood on sampling paper has been developed and validated. The method is straightforward with minimal sample preparation, and is suitable for rural settings. Separation of sulfadoxine, sulfamethoxazole and internal standard was performed using a Purospher STAR RP-18 endcapped LC column (150 x 4.6 mm) with a mobile phase consisting of acetonitrile: sodium acetate buffer pH 5.2, 1=0.1 (1:2, v/v). For sulfadoxine, the within-day precision was 5.3% at 15 mu mol/l and 3.7% at 600 mu mol/l, while for sulfamethoxazole it was 5.7% at 15 mu mol/l and 3.8% at 600 mu mol/l. The tower limit of quantification was determined to 5 mu mol/l and precision was 5.5% and 5.0% for sulfadoxine and sulfamethoxazole, respectively.
Authors
Jenny Lindkvist
Högskolan Dalarna; Kemiteknik
Other publications
>>
Mikaela Malm
Högskolan Dalarna; Kemiteknik
Other publications
>>
Yngve Bergqvist
Högskolan Dalarna; Medicinsk vetenskap
Other publications
>>
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header:
identifier: oai:dalea.du.se:2659
datestamp: 2021-04-15T12:52:49Z
setSpec: SwePub-du
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recordCreationDate: 2007-04-04
identifier:
http://urn.kb.se/resolve?urn=urn:nbn:se:du-2659
10.1016/j.trstmh.2008.11.031
19185326
titleInfo:
@attributes:
lang: eng
title: Straightforward and rapid determination of sulfadoxine and sulfamethoxazole in capillary blood on sampling paper with liquid chromatography and UV detection
abstract: A method for the determination of sulfadoxine and sulfamethoxazole in capillary blood on sampling paper has been developed and validated. The method is straightforward with minimal sample preparation and is suitable for rural settings. Separation of sulfadoxine sulfamethoxazole and internal standard was performed using a Purospher STAR RP-18 endcapped LC column (150 x 4.6 mm) with a mobile phase consisting of acetonitrile: sodium acetate buffer pH 5.2 1=0.1 (1:2 v/v). For sulfadoxine the within-day precision was 5.3% at 15 mu mol/l and 3.7% at 600 mu mol/l while for sulfamethoxazole it was 5.7% at 15 mu mol/l and 3.8% at 600 mu mol/l. The tower limit of quantification was determined to 5 mu mol/l and precision was 5.5% and 5.0% for sulfadoxine and sulfamethoxazole respectively.
subject:
@attributes:
lang: eng
authority: uka.se
topic:
Engineering and Technology
Chemical Engineering
@attributes:
lang: swe
authority: uka.se
topic:
Teknik och teknologier
Kemiteknik
@attributes:
lang: eng
topic: Malaria; Pneumonia; Sulfadoxine; Sulfamethoxazole; Chromatography; Dried blood spots
language:
languageTerm: eng
genre:
publication/journal-article
ref
note:
Published
3
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namePart:
Lindkvist
Jenny
role:
roleTerm: aut
affiliation:
Högskolan Dalarna
Kemiteknik
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type: personal
authority: du
namePart:
Malm
Mikaela
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roleTerm: aut
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Högskolan Dalarna
Kemiteknik
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type: personal
authority: du
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Bergqvist
Yngve
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roleTerm: aut
affiliation:
Högskolan Dalarna
Medicinsk vetenskap
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originInfo:
dateIssued: 2009
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type: host
titleInfo:
title: Transactions of the Royal Society of Tropical Medicine and Hygiene
identifier:
0035-9203
1878-3503
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type: volume
number: 103
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number: 4
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end: 376
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