Straightforward and rapid determination of sulfadoxine and sulfamethoxazole in capillary blood on sampling paper with liquid chromatography and UV detection

Document identifier: oai:dalea.du.se:2659
Access full text here:10.1016/j.trstmh.2008.11.031
Keyword: Engineering and Technology, Chemical Engineering, Teknik och teknologier, Kemiteknik, Malaria; Pneumonia; Sulfadoxine; Sulfamethoxazole; Chromatography; Dried blood spots
Publication year: 2009
Relevant Sustainable Development Goals (SDGs):

The SDG label(s) above have been assigned by OSDG.ai

Abstract:

A method for the determination of sulfadoxine and sulfamethoxazole in capillary blood on sampling paper has been developed and validated. The method is straightforward with minimal sample preparation, and is suitable for rural settings. Separation of sulfadoxine, sulfamethoxazole and internal standard was performed using a Purospher STAR RP-18 endcapped LC column (150 x 4.6 mm) with a mobile phase consisting of acetonitrile: sodium acetate buffer pH 5.2, 1=0.1 (1:2, v/v). For sulfadoxine, the within-day precision was 5.3% at 15 mu mol/l and 3.7% at 600 mu mol/l, while for sulfamethoxazole it was 5.7% at 15 mu mol/l and 3.8% at 600 mu mol/l. The tower limit of quantification was determined to 5 mu mol/l and precision was 5.5% and 5.0% for sulfadoxine and sulfamethoxazole, respectively.

Authors

Jenny Lindkvist

Högskolan Dalarna; Kemiteknik
Other publications >>

Mikaela Malm

Högskolan Dalarna; Kemiteknik
Other publications >>

Yngve Bergqvist

Högskolan Dalarna; Medicinsk vetenskap
Other publications >>

Record metadata

Click to view metadata

	
    header: 
        identifier: oai:dalea.du.se:2659
        datestamp: 2021-04-15T12:52:49Z
        setSpec: SwePub-du
    metadata: 
        mods: 
            @attributes: 
                version: 3.7
            recordInfo: 
                recordContentSource: du
                recordCreationDate: 2007-04-04
            identifier: 
                http://urn.kb.se/resolve?urn=urn:nbn:se:du-2659
                10.1016/j.trstmh.2008.11.031
                19185326
            titleInfo: 
                @attributes: 
                    lang: eng
                title: Straightforward and rapid determination of sulfadoxine and sulfamethoxazole in capillary blood on sampling paper with liquid chromatography and UV detection
            abstract: 
A method for the determination of sulfadoxine and sulfamethoxazole in capillary blood on sampling paper has been developed and validated. The method is straightforward with minimal sample preparation and is suitable for rural settings. Separation of sulfadoxine sulfamethoxazole and internal standard was performed using a Purospher STAR RP-18 endcapped LC column (150 x 4.6 mm) with a mobile phase consisting of acetonitrile: sodium acetate buffer pH 5.2 1=0.1 (1:2 v/v). For sulfadoxine the within-day precision was 5.3% at 15 mu mol/l and 3.7% at 600 mu mol/l while for sulfamethoxazole it was 5.7% at 15 mu mol/l and 3.8% at 600 mu mol/l. The tower limit of quantification was determined to 5 mu mol/l and precision was 5.5% and 5.0% for sulfadoxine and sulfamethoxazole respectively.

            subject: 
                    @attributes: 
                        lang: eng
                        authority: uka.se
                    topic: 
                        Engineering and Technology
                        Chemical Engineering
                    @attributes: 
                        lang: swe
                        authority: uka.se
                    topic: 
                        Teknik och teknologier
                        Kemiteknik
                    @attributes: 
                        lang: eng
                    topic: Malaria; Pneumonia; Sulfadoxine; Sulfamethoxazole; Chromatography; Dried blood spots
            language: 
                languageTerm: eng
            genre: 
                publication/journal-article
                ref
            note: 
                Published
                3
            name: 
                    @attributes: 
                        type: personal
                    namePart: 
                        Lindkvist
                        Jenny
                    role: 
                        roleTerm: aut
                    affiliation: 
                        Högskolan Dalarna
                        Kemiteknik
                    @attributes: 
                        type: personal
                        authority: du
                    namePart: 
                        Malm
                        Mikaela
                    role: 
                        roleTerm: aut
                    affiliation: 
                        Högskolan Dalarna
                        Kemiteknik
                    nameIdentifier: mml
                    @attributes: 
                        type: personal
                        authority: du
                    namePart: 
                        Bergqvist
                        Yngve
                    role: 
                        roleTerm: aut
                    affiliation: 
                        Högskolan Dalarna
                        Medicinsk vetenskap
                    nameIdentifier: ybq
            originInfo: 
                dateIssued: 2009
            relatedItem: 
                @attributes: 
                    type: host
                titleInfo: 
                    title: Transactions of the Royal Society of Tropical Medicine and Hygiene
                identifier: 
                    0035-9203
                    1878-3503
                part: 
                    detail: 
                            @attributes: 
                                type: volume
                            number: 103
                            @attributes: 
                                type: issue
                            number: 4
                    extent: 
                        start: 371
                        end: 376
            physicalDescription: 
                form: print
            typeOfResource: text