The role of phosphorylcreatine in the regulation of mitochondrial respiration in human skeletal muscle.
Document identifier: oai:dalea.du.se:2320
Publication year: 2001Relevant Sustainable Development Goals (SDGs):
The SDG label(s) above have been assigned by OSDG.aiAbstract: 1.
The role of phosphorylcreatine (PCr) and creatine (Cr) in the regulation of mitochondrial respiration was investigated in permeabilised fibre bundles prepared from human vastus lateralis muscle.
2.
Fibre respiration was measured in the absence of ADP (V0) and after sequential additions of submaximal ADP (0.1 mm ADP, Vsubmax), PCr (or Cr) and saturating [ADP] (Vmax).
3.
Vsubmax increased by 55% after addition of saturating creatine (P< 0.01; n = 8) and half the maximal effect was obtained at 5 mm [Cr]. In contrast, Vsubmax decreased by 54% after addition of saturating phosphorylcreatine (P< 0.01; n = 8) and half the maximal effect was obtained at 1 mm [PCr]. Vmax was not affected by Cr or PCr.
4.
Vsubmax was similar when PCr and Cr were added simultaneously at concentrations similar to those in muscle at rest (PCr/Cr = 2) and at low-intensity exercise (PCr/Cr = 0.5). At conditions mimicking high-intensity exercise (PCr/Cr = 0.1), Vsubmax increased to 60% of Vmax (P< 0.01) vs. rest and low-intensity exercise).
5.
Eight of the subjects participated in a 16 day Cr supplementation programme. Following Cr supplementation, V0 decreased by 17% (P< 0.01) vs. prior to Cr supplementation), whereas ADP-stimulated respiration (with and without Cr or PCr) was unchanged.
6.
For the first time evidence is given that PCr is an important regulator of mitochondrial ADP-stimulated respiration. Phosphorylcreatine decreases the sensitivity of mitochondrial respiration to ADP whereas Cr has the opposite effect. During transition from rest to high-intensity exercise, decreases in the PCr/Cr ratio will effectively increase the sensitivity of mitochondrial respiration to ADP. The decrease in V0 after Cr supplementation indicates that intrinsic changes in membrane proton conductance occur.
Authors
Michail Tonkonogi
Högskolan Dalarna; Medicinsk vetenskap
Other publications
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Brandon Walsh
Other publications
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Karin Söderlund
Other publications
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Erik Hultman
Other publications
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Valdur Saks
Other publications
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Kent Sahlin
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identifier: oai:dalea.du.se:2320
datestamp: 2021-04-15T12:57:28Z
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titleInfo:
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lang: eng
title: The role of phosphorylcreatine in the regulation of mitochondrial respiration in human skeletal muscle.
abstract: 1. \t\n\nThe role of phosphorylcreatine (PCr) and creatine (Cr) in the regulation of mitochondrial respiration was investigated in permeabilised fibre bundles prepared from human vastus lateralis muscle.\n2. \t\n\nFibre respiration was measured in the absence of ADP (V0) and after sequential additions of submaximal ADP (0.1 mm ADP Vsubmax) PCr (or Cr) and saturating ADP (Vmax).\n3. \t\n\nVsubmax increased by 55% after addition of saturating creatine (P< 0.01; n = 8) and half the maximal effect was obtained at 5 mm Cr. In contrast Vsubmax decreased by 54% after addition of saturating phosphorylcreatine (P< 0.01; n = 8) and half the maximal effect was obtained at 1 mm PCr. Vmax was not affected by Cr or PCr.\n4. \t\n\nVsubmax was similar when PCr and Cr were added simultaneously at concentrations similar to those in muscle at rest (PCr/Cr = 2) and at low-intensity exercise (PCr/Cr = 0.5). At conditions mimicking high-intensity exercise (PCr/Cr = 0.1) Vsubmax increased to 60% of Vmax (P< 0.01) vs. rest and low-intensity exercise).\n5. \t\n\nEight of the subjects participated in a 16 day Cr supplementation programme. Following Cr supplementation V0 decreased by 17% (P< 0.01) vs. prior to Cr supplementation) whereas ADP-stimulated respiration (with and without Cr or PCr) was unchanged.\n6. \t\n\nFor the first time evidence is given that PCr is an important regulator of mitochondrial ADP-stimulated respiration. Phosphorylcreatine decreases the sensitivity of mitochondrial respiration to ADP whereas Cr has the opposite effect. During transition from rest to high-intensity exercise decreases in the PCr/Cr ratio will effectively increase the sensitivity of mitochondrial respiration to ADP. The decrease in V0 after Cr supplementation indicates that intrinsic changes in membrane proton conductance occur.
language:
languageTerm: eng
genre:
publication/journal-article
ref
note:
Published
6
name:
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namePart:
Tonkonogi
Michail
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roleTerm: aut
affiliation:
Högskolan Dalarna
Medicinsk vetenskap
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0000-0003-1619-9758
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Walsh
Brandon
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Söderlund
Karin
role:
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Hultman
Erik
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Saks
Valdur
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Sahlin
Kent
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originInfo:
dateIssued: 2001
relatedItem:
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titleInfo:
title: Journal of Physiology
identifier:
0022-3751
1469-7793
part:
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type: volume
number: 537
@attributes:
type: issue
number: 3
extent:
start: 971
end: 978
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