Reduced oxidative power but unchanged antioxidative capacity in skeletal muscle from aged humans.
European Journal of Physiology
Document identifier: oai:dalea.du.se:2312
Publication year: 2003Relevant Sustainable Development Goals (SDGs):
The SDG label(s) above have been assigned by OSDG.aiAbstract: The hypothesis that the aging process is associated with mitochondrial dysfunction and oxidative stress has been investigated in human skeletal muscle. Muscle biopsy samples were taken from seven old male subjects [OS; 75 (range 61-86) years] and eight young male subjects [YS; 25 (22-31) years]. Oxidative function was measured both in permeabilised muscle fibres and isolated mitochondria. Despite matching the degree of physical activity, OS had a lower training status than YS as judged from pulmonary maximal O2 consumption (V£O2max, m36%) and handgrip strength (m20%). Both maximal respiration and creatine-stimulated respiration were reduced in muscle fibres from OS (m32 and m34%, respectively). In contrast, respiration in isolated mitochondria was similar in OS and YS. The discrepancy might be explained by a biased harvest of "healthy" mitochondria and/or disruption of structural components during the process of isolation. Cytochrome C oxidase was reduced (m40%, P<0.01), whereas UCP3 protein tended to be elevated in OS (P=0.09). Generation of reactive oxygen species by isolated mitochondria and measures of antioxidative defence (muscle content of glutathione, glutathione redox status, antioxidative enzymes activity) were not significantly different between OS and YS. It is concluded that aging is associated with mitochondrial dysfunction, which appears to be unrelated to reduced physical activity. The hypothesis of increased oxidative stress in aged muscle could not be confirmed in this study.
Authors
Michail Tonkonogi
Högskolan Dalarna; Medicinsk vetenskap
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Maria Fernström
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Brandon Walsh
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Li Li Ji
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Olav Rooyackers
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Folke Hammaqvist
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Jan Wernerman
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Kent Sahlin
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identifier: oai:dalea.du.se:2312
datestamp: 2021-04-15T12:53:03Z
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lang: eng
title: Reduced oxidative power but unchanged antioxidative capacity in skeletal muscle from aged humans.
abstract: The hypothesis that the aging process is associated with mitochondrial dysfunction and oxidative stress has been investigated in human skeletal muscle. Muscle biopsy samples were taken from seven old male subjects OS; 75 (range 61-86) years and eight young male subjects YS; 25 (22-31) years. Oxidative function was measured both in permeabilised muscle fibres and isolated mitochondria. Despite matching the degree of physical activity OS had a lower training status than YS as judged from pulmonary maximal O2 consumption (V£O2max m36%) and handgrip strength (m20%). Both maximal respiration and creatine-stimulated respiration were reduced in muscle fibres from OS (m32 and m34% respectively). In contrast respiration in isolated mitochondria was similar in OS and YS. The discrepancy might be explained by a biased harvest of "healthy" mitochondria and/or disruption of structural components during the process of isolation. Cytochrome C oxidase was reduced (m40% P<0.01) whereas UCP3 protein tended to be elevated in OS (P=0.09). Generation of reactive oxygen species by isolated mitochondria and measures of antioxidative defence (muscle content of glutathione glutathione redox status antioxidative enzymes activity) were not significantly different between OS and YS. It is concluded that aging is associated with mitochondrial dysfunction which appears to be unrelated to reduced physical activity. The hypothesis of increased oxidative stress in aged muscle could not be confirmed in this study.
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Published
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Michail
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Högskolan Dalarna
Medicinsk vetenskap
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Fernström
Maria
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Walsh
Brandon
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Li Li
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Rooyackers
Olav
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Hammaqvist
Folke
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Wernerman
Jan
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Kent
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dateIssued: 2003
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subTitle: European Journal of Physiology
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1432-2013
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