Reduced oxidative power but unchanged antioxidative capacity in skeletal muscle from aged humans.

European Journal of Physiology

Document identifier: oai:dalea.du.se:2312
Publication year: 2003
Relevant Sustainable Development Goals (SDGs):

The SDG label(s) above have been assigned by OSDG.ai

Abstract:

The hypothesis that the aging process is associated with mitochondrial dysfunction and oxidative stress has been investigated in human skeletal muscle. Muscle biopsy samples were taken from seven old male subjects [OS; 75 (range 61-86) years] and eight young male subjects [YS; 25 (22-31) years]. Oxidative function was measured both in permeabilised muscle fibres and isolated mitochondria. Despite matching the degree of physical activity, OS had a lower training status than YS as judged from pulmonary maximal O2 consumption (V£O2max, m36%) and handgrip strength (m20%). Both maximal respiration and creatine-stimulated respiration were reduced in muscle fibres from OS (m32 and m34%, respectively). In contrast, respiration in isolated mitochondria was similar in OS and YS. The discrepancy might be explained by a biased harvest of "healthy" mitochondria and/or disruption of structural components during the process of isolation. Cytochrome C oxidase was reduced (m40%, P<0.01), whereas UCP3 protein tended to be elevated in OS (P=0.09). Generation of reactive oxygen species by isolated mitochondria and measures of antioxidative defence (muscle content of glutathione, glutathione redox status, antioxidative enzymes activity) were not significantly different between OS and YS. It is concluded that aging is associated with mitochondrial dysfunction, which appears to be unrelated to reduced physical activity. The hypothesis of increased oxidative stress in aged muscle could not be confirmed in this study.

Authors

Michail Tonkonogi

Högskolan Dalarna; Medicinsk vetenskap
Other publications >>

Maria Fernström

Other publications >>

Brandon Walsh

Other publications >>

Li Li Ji

Other publications >>

Olav Rooyackers

Other publications >>

Folke Hammaqvist

Other publications >>

Jan Wernerman

Other publications >>

Kent Sahlin

Other publications >>

Record metadata

Click to view metadata

	
    header: 
        identifier: oai:dalea.du.se:2312
        datestamp: 2021-04-15T12:53:03Z
        setSpec: SwePub-du
    metadata: 
        mods: 
            @attributes: 
                version: 3.7
            recordInfo: 
                recordContentSource: du
                recordCreationDate: 2006-10-02
            identifier: http://urn.kb.se/resolve?urn=urn:nbn:se:du-2312
            titleInfo: 
                @attributes: 
                    lang: eng
                title: Reduced oxidative power but unchanged antioxidative capacity in skeletal muscle from aged humans.
            abstract: 
The hypothesis that the aging process is associated with mitochondrial dysfunction and oxidative stress has been investigated in human skeletal muscle. Muscle biopsy samples were taken from seven old male subjects OS; 75 (range 61-86) years and eight young male subjects YS; 25 (22-31) years. Oxidative function was measured both in permeabilised muscle fibres and isolated mitochondria. Despite matching the degree of physical activity OS had a lower training status than YS as judged from pulmonary maximal O2 consumption (V£O2max m36%) and handgrip strength (m20%). Both maximal respiration and creatine-stimulated respiration were reduced in muscle fibres from OS (m32 and m34% respectively). In contrast respiration in isolated mitochondria was similar in OS and YS. The discrepancy might be explained by a biased harvest of "healthy" mitochondria and/or disruption of structural components during the process of isolation. Cytochrome C oxidase was reduced (m40% P<0.01) whereas UCP3 protein tended to be elevated in OS (P=0.09). Generation of reactive oxygen species by isolated mitochondria and measures of antioxidative defence (muscle content of glutathione glutathione redox status antioxidative enzymes activity) were not significantly different between OS and YS. It is concluded that aging is associated with mitochondrial dysfunction which appears to be unrelated to reduced physical activity. The hypothesis of increased oxidative stress in aged muscle could not be confirmed in this study.

            language: 
                languageTerm: eng
            genre: 
                publication/journal-article
                ref
            note: 
                Published
                8
            name: 
                    @attributes: 
                        type: personal
                        authority: du
                    namePart: 
                        Tonkonogi
                        Michail
                    role: 
                        roleTerm: aut
                    affiliation: 
                        Högskolan Dalarna
                        Medicinsk vetenskap
                    nameIdentifier: 
                        mtn
                        0000-0003-1619-9758
                    @attributes: 
                        type: personal
                    namePart: 
                        Fernström
                        Maria
                    role: 
                        roleTerm: aut
                    @attributes: 
                        type: personal
                    namePart: 
                        Walsh
                        Brandon
                    role: 
                        roleTerm: aut
                    @attributes: 
                        type: personal
                    namePart: 
                        Ji
                        Li Li
                    role: 
                        roleTerm: aut
                    @attributes: 
                        type: personal
                    namePart: 
                        Rooyackers
                        Olav
                    role: 
                        roleTerm: aut
                    @attributes: 
                        type: personal
                    namePart: 
                        Hammaqvist
                        Folke
                    role: 
                        roleTerm: aut
                    @attributes: 
                        type: personal
                    namePart: 
                        Wernerman
                        Jan
                    role: 
                        roleTerm: aut
                    @attributes: 
                        type: personal
                    namePart: 
                        Sahlin
                        Kent
                    role: 
                        roleTerm: aut
            originInfo: 
                dateIssued: 2003
            relatedItem: 
                @attributes: 
                    type: host
                titleInfo: 
                    title: Pflügers Archiv
                    subTitle: European Journal of Physiology
                identifier: 
                    0031-6768
                    1432-2013
                part: 
                    detail: 
                            @attributes: 
                                type: volume
                            number: 446
                            @attributes: 
                                type: issue
                            number: 2
                    extent: 
                        start: 261
                        end: 269
            physicalDescription: 
                form: print
            typeOfResource: text