Correlating gene expression to physiological parameters and environmental conditions during cold acclimation of Pinus sylvestris, identification of molecular markers using cDNA microarrays

Document identifier: oai:dalea.du.se:1901
Access full text here:10.1093/treephys/26.10.1297
Keyword: Agricultural Sciences, Agricultural Science, Forestry and Fisheries, Lantbruksvetenskaper, Lantbruksvetenskap, skogsbruk och fiske, Apical buds; climate room; field; freezing tolerance; gene expression; mRNA level; root electrolyte leakage; Scots pine; seedlings; shoot electrolyte leakage; transcript abundance
Publication year: 2006
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Abstract:

Scots pine (Pinus sylvestris L.) seedlings were grown under different conditions (three field locations, two seasons and two climate room regimes), and then analyzed for freezing tolerance of shoots and roots and for transcript abundance in apical buds based on a cDNA microarray containing about 1500 expressed sequence tags (ESTs) from buds of cold-treated Scots pine seedlings. In a climate room providing long daily photoperiods and high temperatures, seedlings did not develop freezing tolerance, whereas seedlings in a climate room set to provide declining temperatures and day lengths developed moderate freezing tolerance. Control seedlings grown outside under field conditions developed full freezing tolerance. Differences in physiological behavior of the different seedling groups, combined with molecular analysis, allowed identification of a large group of genes, expression of which changed during the development of freezing tolerance. Transcript abundance of several of these genes was highly correlated with freezing tolerance in seedlings differing in provenance, field location or age, making them excellent candidate marker genes for molecular tests for freezing tolerance.

Authors

R.V.L. Joosen

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M. Lammers

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P. Balk

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P. Brönnum

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M.C.J.M. Konings

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M. Perks

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Eva Stattin

Högskolan Dalarna; Träteknologi
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M.F. van Wordragen

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L.H.M. Geest

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    header: 
        identifier: oai:dalea.du.se:1901
        datestamp: 2021-04-15T13:12:47Z
        setSpec: SwePub-du
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                version: 3.7
            recordInfo: 
                recordContentSource: du
                recordCreationDate: 2006-03-07
            identifier: 
                http://urn.kb.se/resolve?urn=urn:nbn:se:du-1901
                10.1093/treephys/26.10.1297
            titleInfo: 
                @attributes: 
                    lang: eng
                title: Correlating gene expression to physiological parameters and environmental conditions during cold acclimation of Pinus sylvestris identification of molecular markers using cDNA microarrays
            abstract: 
Scots pine (Pinus sylvestris L.) seedlings were grown under different conditions (three field locations two seasons and two climate room regimes) and then analyzed for freezing tolerance of shoots and roots and for transcript abundance in apical buds based on a cDNA microarray containing about 1500 expressed sequence tags (ESTs) from buds of cold-treated Scots pine seedlings. In a climate room providing long daily photoperiods and high temperatures seedlings did not develop freezing tolerance whereas seedlings in a climate room set to provide declining temperatures and day lengths developed moderate freezing tolerance. Control seedlings grown outside under field conditions developed full freezing tolerance. Differences in physiological behavior of the different seedling groups combined with molecular analysis allowed identification of a large group of genes expression of which changed during the development of freezing tolerance. Transcript abundance of several of these genes was highly correlated with freezing tolerance in seedlings differing in provenance field location or age making them excellent candidate marker genes for molecular tests for freezing tolerance.

            subject: 
                    @attributes: 
                        lang: eng
                        authority: uka.se
                    topic: 
                        Agricultural Sciences
                        Agricultural Science Forestry and Fisheries
                    @attributes: 
                        lang: swe
                        authority: uka.se
                    topic: 
                        Lantbruksvetenskaper
                        Lantbruksvetenskap skogsbruk och fiske
                    @attributes: 
                        lang: eng
                    topic: apical buds; climate room; field; freezing tolerance; gene expression; mRNA level; root electrolyte leakage; Scots pine; seedlings; shoot electrolyte leakage; transcript abundance
            language: 
                languageTerm: eng
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                publication/journal-article
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                Published
                9
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                        Joosen
                        R.V.L.
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                        Lammers
                        M.
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                        Balk
                        P.
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                        Brönnum
                        P.
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                        Konings
                        M.C.J.M.
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                        Stattin
                        Eva
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                    affiliation: 
                        Högskolan Dalarna
                        Träteknologi
                    nameIdentifier: esa
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                        van Wordragen
                        M.F.
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                dateIssued: 2006
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                titleInfo: 
                    title: Tree Physiology
                identifier: 
                    0829-318X
                    1758-4469
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                    detail: 
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                                type: volume
                            number: 26
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                            number: 10
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