Development and validation of a bioanalytical method using automated solid-phase extraction and LC-UV for the simultaneous determination of lumefantrine and its desbutyl metabolite in plasma
Document identifier: oai:dalea.du.se:1202
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10.1016/j.jpba.2004.07.041Keyword: Engineering and Technology,
Chemical Engineering,
Teknik och teknologier,
Kemiteknik,
Antimalarial; lumefantrine; lipophilic; liquid chromatography; solid-phase extraction; stability; validationPublication year: 2005Relevant Sustainable Development Goals (SDGs):
The SDG label(s) above have been assigned by OSDG.aiAbstract: A bioanalytical method for the determination of lumefantrine (LF) and its metabolite desbutyl-lumefantrine (DLF) in plasma by solid-phase extraction (SPE) and liquid chromatography has been developed. Plasma proteins were precipitated with acetonitrile: acetic acid (99:1, v/v) containing a DLF analogue internal standard before being loaded onto a octylsilica (3 M Empore) SPE column. Two different DLF analogues were evaluated as internal standards. The compounds were analysed by liquid chromatography UV detection on a SB-CN (250 mm x 4.6 mm) column with a mobile phase containing acetonitrile-sodium phosphate buffer pH (2.0; 0.1 M) (55:45, v/v) and sodium perchlorate 0.05 M. Different SPE columns were evaluated during method development to optimise reproducibility and recovery for LF, DLF and the two different DLF analogues. The within-day precisions for LF were 6.6 and 2.1% at 0.042 and 8.02 μ g/mL, respectively, and for DLF 4.5 and 1.5% at 0.039 and 0.777 μ g/mL, respectively. The between-day precisions for LF were 12.0 and 2.9% at 0.042 and 8.02 μ g/mL, respectively, while for DLF 0.7 and 1.2% at 0.039 and 0.777 μ g/mL, respectively. The limit of quantification was 0.024 and 0.021 μ g/mL for LF and DLF, respectively. Different amounts of lipids in plasma did not affect the absolute recovery of LF or DLF.
Authors
Niklas Lindegårdh
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A. Annerberg
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Daniel Blessborn
Högskolan Dalarna; Kemiteknik
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Yngve Bergqvist
Högskolan Dalarna; Kemiteknik
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N. Day
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N.J. White
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header:
identifier: oai:dalea.du.se:1202
datestamp: 2021-04-15T13:01:52Z
setSpec: SwePub-du
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http://urn.kb.se/resolve?urn=urn:nbn:se:du-1202
10.1016/j.jpba.2004.07.041
15862688
titleInfo:
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lang: eng
title: Development and validation of a bioanalytical method using automated solid-phase extraction and LC-UV for the simultaneous determination of lumefantrine and its desbutyl metabolite in plasma
abstract: A bioanalytical method for the determination of lumefantrine (LF) and its metabolite desbutyl-lumefantrine (DLF) in plasma by solid-phase extraction (SPE) and liquid chromatography has been developed. Plasma proteins were precipitated with acetonitrile: acetic acid (99:1 v/v) containing a DLF analogue internal standard before being loaded onto a octylsilica (3 M Empore) SPE column. Two different DLF analogues were evaluated as internal standards. The compounds were analysed by liquid chromatography UV detection on a SB-CN (250 mm x 4.6 mm) column with a mobile phase containing acetonitrile-sodium phosphate buffer pH (2.0; 0.1 M) (55:45 v/v) and sodium perchlorate 0.05 M. Different SPE columns were evaluated during method development to optimise reproducibility and recovery for LF DLF and the two different DLF analogues. The within-day precisions for LF were 6.6 and 2.1% at 0.042 and 8.02 μ g/mL respectively and for DLF 4.5 and 1.5% at 0.039 and 0.777 μ g/mL respectively. The between-day precisions for LF were 12.0 and 2.9% at 0.042 and 8.02 μ g/mL respectively while for DLF 0.7 and 1.2% at 0.039 and 0.777 μ g/mL respectively. The limit of quantification was 0.024 and 0.021 μ g/mL for LF and DLF respectively. Different amounts of lipids in plasma did not affect the absolute recovery of LF or DLF.
subject:
@attributes:
lang: eng
authority: uka.se
topic:
Engineering and Technology
Chemical Engineering
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lang: swe
authority: uka.se
topic:
Teknik och teknologier
Kemiteknik
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lang: eng
topic: antimalarial; lumefantrine; lipophilic; liquid chromatography; solid-phase extraction; stability; validation
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Published
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Lindegårdh
Niklas
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Annerberg
A.
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Daniel
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Högskolan Dalarna
Kemiteknik
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Yngve
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Kemiteknik
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title: Journal of Pharmaceutical and Biomedical Analysis
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1873-264X
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number: 37
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