Valorization of waste forest biomass toward the production of cello-oligosaccharides with potential prebiotic activity by utilizing customized enzyme cocktails

Document identifier: oai:DiVA.org:ltu-78018
Access full text here:10.1186/s13068-019-1628-z
Keyword: Engineering and Technology, Industrial Biotechnology, Bioprocess Technology, Teknik och teknologier, Industriell bioteknik, Bioprocessteknik, Non-digestible oligosaccharides, Cellobiose, Processive endoglucanases, Prebiotics, Enzyme hydrolysis, Nanofiltration, Biokemisk processteknik, Biochemical Process Engineering
Publication year: 2019
Relevant Sustainable Development Goals (SDGs):
SDG 9 Industry, innovation and infrastructureSDG 7 Affordable and clean energy
The SDG label(s) above have been assigned by OSDG.ai

Abstract:

Background

Production of value-added materials from lignocellulosic biomass residues is an emerging sector that has attracted much attention as it offers numerous benefits from an environmental and economical point of view. Non-digestible oligosaccharides represent a group of carbohydrates that are resistant to gastrointestinal digestion, and therefore, they are considered as potential prebiotic candidates. Such oligosaccharides can derive from the biomass cellulose fraction through a controlled enzymatic hydrolysis that eliminates the yield of monomers.

Results

In the present study, hydrolysis of organosolv-pretreated forest residues (birch and spruce) was tested in the presence of four cellulases (EG5, CBH7, CBH6, EG7) and one accessory enzyme (LPMO). The optimal enzyme combinations were comprised of 20% EG5, 43% CBH7, 22% TtLPMO, 10% PaCbh6a and 5% EG7 in the case of birch and 35% EG5, 45% CBH7, 10% TtLPMO, 10% PaCbh6a and 5% EG7 in the case of spruce, leading to 22.3% and 19.1 wt% cellulose conversion into cellobiose, respectively. Enzymatic hydrolysis was applied on scale-up reactions, and the produced oligosaccharides (consisted of > 90% cellobiose) were recovered and separated from glucose through nanofiltration at optimized temperature (50 °C) and pressure (10 bar) conditions, yielding a final product with cellobiose-to-glucose ratio of 21.1 (birch) and 20.2 (spruce). Cellobiose-rich hydrolysates were tested as fermentative substrates for different lactic acid bacteria. It was shown that they can efficiently stimulate the growth of two Lactobacilli strains.

Conclusions

Controlled enzymatic hydrolysis with processive cellulases, combined with product recovery and purification, as well as enzyme recycling can potentially support the sustainable production of food-grade oligosaccharides from forest biomass.

Authors

Anthi C. Karnaouri

Luleå tekniska universitet; Kemiteknik
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Leonidas Matsakas

Luleå tekniska universitet; Kemiteknik
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Eleni Krikigianni

Luleå tekniska universitet; Kemiteknik
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Ulrika Rova

Luleå tekniska universitet; Kemiteknik
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Paul Christakopoulos

Luleå tekniska universitet; Kemiteknik
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