Tailoring Celluclast (R) Cocktail's Performance towards the Production of Prebiotic Cello-Oligosaccharides from Waste Forest Biomass

Document identifier: oai:DiVA.org:ltu-77851
Access full text here:10.3390/catal9110897
Keyword: Engineering and Technology, Industrial Biotechnology, Bioprocess Technology, Teknik och teknologier, Industriell bioteknik, Bioprocessteknik, Non-digestible oligosaccharides, Celluclast®, Cellobiose, Conduritol-B-epoxide, Prebiotic, Lignocellulose enzyme hydrolysis, Biokemisk processteknik, Biochemical Process Engineering
Publication year: 2019
Relevant Sustainable Development Goals (SDGs):
SDG 12 Responsible consumption and productionSDG 9 Industry, innovation and infrastructureSDG 7 Affordable and clean energy
The SDG label(s) above have been assigned by OSDG.ai

Abstract:

The main objective of this study focused on the sustainable production of cellobiose and other cellulose-derived oligosaccharides from non-edible sources, more specifically, from forest residues. For this purpose, a fine-tuning of the performance of the commercially available enzyme mixture Celluclast® was conducted towards the optimization of cellobiose production. By enzyme reaction engineering (pH, multi-stage hydrolysis with buffer exchange, addition of β-glucosidase inhibitor), a cellobiose-rich product with a high cellobiose to glucose ratio (37.4) was achieved by utilizing organosolv-pretreated birch biomass. In this way, controlled enzymatic hydrolysis combined with efficient downstream processing, including product recovery and purification through ultrafiltration and nanofiltration, can potentially support the sustainable production of food-grade oligosaccharides from forest biomass. The potential of the hydrolysis product to support the growth of two Lactobacilli probiotic strains as a sole carbon source was also demonstrated

Authors

Anthi C. Karnaouri

Luleå tekniska universitet; Kemiteknik
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Leonidas Matsakas

Luleå tekniska universitet; Kemiteknik
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Saskja Bühler

Luleå tekniska universitet; Kemiteknik
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Madhu Nair Muraleedharan

Luleå tekniska universitet; Kemiteknik
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Paul Christakopoulos

Luleå tekniska universitet; Kemiteknik
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Ulrika Rova

Luleå tekniska universitet; Kemiteknik
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