Simultaneous production of DHA and squalene from Aurantiochytrium sp. grown on forest biomass hydrolysates
Document identifier: oai:DiVA.org:ltu-76775
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10.1186/s13068-019-1593-6Keyword: Engineering and Technology,
Industrial Biotechnology,
Bioprocess Technology,
Teknik och teknologier,
Industriell bioteknik,
Bioprocessteknik,
Thraustochytrids,
Aurantiochytrium sp.,
DHA,
Squalene,
Wood biomass,
Organosolv pretreatment,
Heterotrophic growth,
Lipid production,
Biokemisk processteknik,
Biochemical Process EngineeringPublication year: 2019Relevant Sustainable Development Goals (SDGs):
The SDG label(s) above have been assigned by OSDG.aiAbstract: Background
Recent evidence points to the nutritional importance of docosahexaenoic acid (DHA) in the human diet. Thraustochytrids are heterotrophic marine oleaginous microorganisms capable of synthesizing high amounts of DHA, as well as other nutraceutical compounds such as squalene, in their cellular compartment. Squalene is a natural triterpene and an important biosynthetic precursor to all human steroids. It has a wide range of applications in the cosmetic and pharmaceutical industries, with benefits that include boosting immunity and antioxidant activity. Apart from its nutritional quality, it can also be utilized for high-grade bio-jet fuel by catalytic conversion.
Results
In the present study, the potential of thraustochytrid strain Aurantiochytrium sp. T66 to produce DHA and squalene was evaluated. When the strain was cultivated on organosolv-pretreated birch hydrolysate (30 g/L glucose) in flask, it resulted in 10.39 g/L of cell dry weight and 4.98 g/L of total lipids, of which 25.98% was DHA. In contrast, when the strain was grown in a bioreactor, cell dry weight, total lipid, and DHA increased to 11.24 g/L, 5.90 g/L, and 35.76%, respectively. The maximum squalene yield was 69.31 mg/gCDW (0.72 g/L) when the strain was cultivated in flask, but it increased to 88.47 mg/gCDW (1.0 g/L), when cultivation shifted to a bioreactor.
Conclusions
This is the first report demonstrating the utilization of low cost non-edible lignocellulosic feedstock to cultivate the marine oleaginous microorganism Aurantiochytrium sp. for the production of nutraceutical vital compounds. Owing to the simultaneous generation of DHA and squalene, the strain is suitable for industrial-scale production of nutraceuticals.
Authors
Alok Patel
Luleå tekniska universitet; Kemiteknik
Other publications
>>
Ulrika Rova
Luleå tekniska universitet; Kemiteknik
Other publications
>>
Paul Christakopoulos
Luleå tekniska universitet; Kemiteknik
Other publications
>>
Leonidas Matsakas
Luleå tekniska universitet; Kemiteknik
Other publications
>>
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identifier: oai:DiVA.org:ltu-76775
datestamp: 2021-04-19T12:40:39Z
setSpec: SwePub-ltu
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version: 3.7
recordInfo:
recordContentSource: ltu
recordCreationDate: 2019-11-20
identifier:
http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-76775
10.1186/s13068-019-1593-6
31687043
2-s2.0-85074448972
titleInfo:
@attributes:
lang: eng
title: Simultaneous production of DHA and squalene from Aurantiochytrium sp. grown on forest biomass hydrolysates
abstract: Background
Recent evidence points to the nutritional importance of docosahexaenoic acid (DHA) in the human diet. Thraustochytrids are heterotrophic marine oleaginous microorganisms capable of synthesizing high amounts of DHA as well as other nutraceutical compounds such as squalene in their cellular compartment. Squalene is a natural triterpene and an important biosynthetic precursor to all human steroids. It has a wide range of applications in the cosmetic and pharmaceutical industries with benefits that include boosting immunity and antioxidant activity. Apart from its nutritional quality it can also be utilized for high-grade bio-jet fuel by catalytic conversion.
Results
In the present study the potential of thraustochytrid strain Aurantiochytrium sp. T66 to produce DHA and squalene was evaluated. When the strain was cultivated on organosolv-pretreated birch hydrolysate (30 g/L glucose) in flask it resulted in 10.39 g/L of cell dry weight and 4.98 g/L of total lipids of which 25.98% was DHA. In contrast when the strain was grown in a bioreactor cell dry weight total lipid and DHA increased to 11.24 g/L 5.90 g/L and 35.76% respectively. The maximum squalene yield was 69.31 mg/gCDW (0.72 g/L) when the strain was cultivated in flask but it increased to 88.47 mg/gCDW (1.0 g/L) when cultivation shifted to a bioreactor.
Conclusions
This is the first report demonstrating the utilization of low cost non-edible lignocellulosic feedstock to cultivate the marine oleaginous microorganism Aurantiochytrium sp. for the production of nutraceutical vital compounds. Owing to the simultaneous generation of DHA and squalene the strain is suitable for industrial-scale production of nutraceuticals.
subject:
@attributes:
lang: eng
authority: uka.se
topic:
Engineering and Technology
Industrial Biotechnology
Bioprocess Technology
@attributes:
lang: swe
authority: uka.se
topic:
Teknik och teknologier
Industriell bioteknik
Bioprocessteknik
@attributes:
lang: eng
topic: Thraustochytrids
@attributes:
lang: eng
topic: Aurantiochytrium sp.
@attributes:
lang: eng
topic: DHA
@attributes:
lang: eng
topic: Squalene
@attributes:
lang: eng
topic: Wood biomass
@attributes:
lang: eng
topic: Organosolv pretreatment
@attributes:
lang: eng
topic: Heterotrophic growth
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lang: eng
topic: Lipid production
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lang: swe
authority: ltu
topic: Biokemisk processteknik
genre: Research subject
@attributes:
lang: eng
authority: ltu
topic: Biochemical Process Engineering
genre: Research subject
language:
languageTerm: eng
genre:
publication/journal-article
ref
note:
Published
4
Validerad;2019;Nivå 2;2019-11-20 (johcin)
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Alok
Dr.
1989-
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Luleå tekniska universitet
Kemiteknik
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Ulrika
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Luleå tekniska universitet
Kemiteknik
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Leonidas
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affiliation:
Luleå tekniska universitet
Kemiteknik
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0000-0002-3687-6173
originInfo:
dateIssued: 2019
publisher: BioMed Central
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titleInfo:
title: Biotechnology for Biofuels
identifier:
1754-6834
1754-6834
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@attributes:
type: volume
number: 12
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type: issue
number: 1
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type: artNo
number: 255
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